AB0038 CIRCULATING MICRORNAS IN HAND OSTEOARTHRITIS

Background: microRNAs (miRNAs) are small non-coding RNAs that can ignite the degradation of mRNAs or inhibit protein translation and therefore essential for several physiological pathological functions. miRNAs regulate up to 60 % human mRNA, including genes related cartilage development, homeostasis, OA pathology. For example, miR-9 inhibits matrix metalloproteinase 13 miR-140 expression level correlates with disease progression knee (Zhang et al.; Chao al.). Under certain circumstances, be released into body fluids easily detected in blood samples. Therefore, hot candidates as biomarkers early diagnosis structural OA. Objectives: The aim this study was evaluate circulating patients hand osteoarthritis (HOA) healthy individuals. Simultaneously, we studied specific order differentiate between erosive non-erosive subsets disease. Methods: Eight HOA (erosive: n=4, 3 females, mean age=63.7±7 yrs; non-erosive: age= 62.4±6 yrs) 4 controls (3 age=63.5±7 were included study. Firstly, Advance TaqMan low-density assay (TLDA) performed purpose miRNA high-throughput screening. Differently expressed further verified by real-time qPCR on validation cohort 31 (19 age=66.2±7 yrs, erosive: n=9, n=10, controls: n=12). Results: TLDA profiling displayed 346 plasma controls. We demonstrated 40 differently compared Using a qPCR, increased levels 10 controls, e.g. miR-191-5p (3.4 fold), miR-151a-3p fold) miR-222-3p (2.4 fold). did not find any miRNA, which could distinct from subset Conclusion: Extensive revealed associated help better understand pathogenesis. References: [1]Chao, Yu, al. “Expression MiR-140 MiR-199 Synovia Its Correlation Progression Knee Osteoarthritis.” Medical Science Monitor , vol. 26, 2020, pp. 1–6, doi:10.12659/MSM.918174. [2]Zhang, Hongxin, “Downregulation MicroRNA-9 Increases Matrix Metalloproteinase-13 Expression Levels Facilitates Osteoarthritis Onset.” Molecular Medicine Reports 17, no. 3, 2018, 3708–14, doi:10.3892/mmr.2017.8340. Acknowledgements: Supported AZV NV18-01-00542, MHCR No. 023728 Disclosure Interests: None declared